Isolation and Screening of Actinomycetes from Soil Sample of Dal Lake (Kashmir) Against Selected Pathogens

Actinomycetes are considered as one of the most diverse groups of filamentous bacteria capable of thriving into different types of ecological niches due to their bioactive potential. The main objective of the present study was Isolation and Screening of Actinomycetes from soil of Dal Lake of Kashmir region and characterized for morphological identification and evaluated for antibacterial activity against human pathogens. Total 50 Actinomycetes were isolated and characterized for morphological identification and evaluated for antibacterial activity. Out of these isolated, five actinomycetes showed antimicrobial activity against selected bacterial pathogens. Screening using agar diffusion assay was carried out and total five actinomycetes isolated were found active against MTCC cultures Including Bacillus subtilis (MTCC 441), Pseudomonas aeruginosa (MTCC 4673), Staphylococcus aureus (MTCC 3160), Pseudomonas fluorescens (MTCC 103),Mycobacterium smegmatis (MTCC 994), E. coli (MTCC 443) and Streptococcus mutans (MTCC 890). The study indicated that Dal Lake of Kashmir had diverse group of actinomycetes with broad spectrum antimicrobial activity.